These experiments were conducted to develop rapid methods for the measurement of EFE activity and antioxidant effect and to screen chemicals which show inhibition of EFE activity and/or antioxidant effect with these methods. The results are summarized as follows:
1. The screening methods of chemicals capable of inhibiting EFE activity and/or having antioxidant effect, which were developed in this experiment were more rapid and accurate than evaluation of chemical effects by the direct addition to vasal solution.
2. Allium sativum extracts. 8-HQS(8-hydroxy quinoline sulfate), n-propylgallate, BHA (butylated hydroxyanisole), and benzoic acid considerably inhibited EFE activity when apple flesh of preclimacteric phase was used as a reaction material.
3. 8-HQS, galangin, epicatechin, quercetin, rutin, n-propylgallate, BHT(butylated hydroxytoluene), STS(silver thiosulfate), Rubus crataegifolius extracts, and benzoic acid showed fairly good antioxidant effect.
4. The treatments of 8-HQS, benzoic acid, and n-propylgallate were effective in both inhibition of EFE activity and antioxidant effect, and the results of this experiment were strongly supported by their physiological phenomena.
5. Because Allium sativum and Rubus crataegifolius as natural extracts showed possible capabilities in both inhibition of EFE activity and antioxidant effect, they need to be analyzed and characterized further.
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